pMK297 (DHC1-mAID Hygro)

LOCUS       pMK297 (DHC1-mAID Hygro)              9446 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  DHC1 tagging with mAID.
ACCESSION   .
VERSION     .
KEYWORDS    pMK297 (DHC1-mAID Hygro)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano 
            R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, 
            Hayashi KI, Kanemaki MT.
  TITLE     The auxin-inducible degron 2 technology provides sharp degradation 
            control in yeast, mammalian cells, and mice.
  JOURNAL   Nat Commun (2020) 11(1):5701
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1314..1517
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             1524..2243
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1569..1590)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2177..2198
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2361..2482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2398..2417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2452..2471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(2513..2546)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        2607..3106
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2642..2665)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3039..3058
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3167..4186
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     protein_bind    complement(4749..4782)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(5879..5898)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5880..5898)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5905..5922)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5905..5921)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5914..5936)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6062..6490
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6149..6168)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6359..6380
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             6834..7628
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6888..6907)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7498..7517
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             7646..8506
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7864..7883)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8677..9265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9166..9185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9419..9436
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccttcag cgagaggatc aaacagctgc agaacatctc actggcagct gcatctggtg
      301 gcgccaagga gctaaaggtg aaggcgctcc tgacgagtct cgggtggtca gcagctgtcc
      361 tgggctgggg tgggagtggc tctggggaaa aacacagggc ccaggtctga cctgagctcc
      421 ttcccctggg ggctgctgct ttccacagaa catccacgtg tgcctgggtg gcctgttcgt
      481 gcctgaggcg tacatcactg ccaccaggca gtatgtggcc caggccaaca gctggtccct
      541 ggaggagctc tgcctggaag tcaacgtcac cacctcacag ggcgccaccc ttgacgcttg
      601 cagcttcgga gtcacgggtg agtggagtct cacagaaaat actggctctt tgcaggtgac
      661 ctcggtggcc tgagaccatt gttcccagat acatgcactt agggtgaccg gctggcagtt
      721 gggtggagcc tctgggcgcc ctgtgactgg ggtttgtgta gctgttgtgt tcacctcagc
      781 ctgggttttg gcttccgcct cacaggtttg aaacttcaag gggccacgtg caacaacaac
      841 aagctgtcac tgtccaatgc catctcaacc gcccttcccc tgacgcagct gcgctgggtc
      901 aagcagacaa acaccgagaa gaaggccagt gtggtaagga ggcactgcct ttcccaggca
      961 ttctgcaggg acccctgcgg taacaagggc agaggcggct cctcttctat gcctgggttc
     1021 cacttggaac gggaaatgag gttcacagag gaaatccatt tcgcacctgt ccaaacctct
     1081 ccttgccggg ccccatcagc tgtcccgggc agtcttccag ttttcttact tttcccttaa
     1141 gccaccagta aacccctctg cttctgcagg taaccttacc tgtctacctg aacttcaccc
     1201 gtgcagacct catcttcacc gtggacttcg aaattgctac aaaggaggac cctcgctcgt
     1261 tctacgaaag aggtgtcgca gtcttgtgca cagagggatc cggtgcaggc gccaaggaga
     1321 agagtgcttg tcctaaagat ccagccaaac ctccggccaa ggcacaagtt gtgggatggc
     1381 caccggtgag atcataccgg aagaacgtga tggtttcctg ccaaaaatca agcggtggcc
     1441 cggaggcggc ggcgttcgtg aaggtatcaa tggacggagc accgtacttg aggaaaatcg
     1501 atttgaggat gtataaagct agcatggtga gcaagggcga ggagctgttc accggggtgg
     1561 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg
     1621 agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca
     1681 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca
     1741 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1801 acgtccagga gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg
     1861 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1921 aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata
     1981 tcacggccga caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg
     2041 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2101 ccgtgctgct gcccgacaac cactacctga gccatcagtc caagctgagc aaagacccca
     2161 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg attacacatg
     2221 gcatggacga gctgtacaag taatctagat aactgatcat aatcagccat accacatttg
     2281 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2341 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2461 ccaaactcat caatgtatct taacgcgtcg atcatattca ataaccctta atataacttc
     2521 gtataatgta tgctatacga agttattagg tctgaagagg agtttacgtc cagccaagct
     2581 taggatctcg acctcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     2641 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     2701 cacacattcc acatccaccg gtaggcgcca accgactccg ttctttggtg gccccttcgc
     2761 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     2821 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     2881 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     2941 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     3001 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     3061 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct
     3121 agatctcgag cagctgaagc ttaccgctag catggataga tccggaaagc ctgaactcac
     3181 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     3241 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     3301 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     3361 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     3421 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     3481 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     3541 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     3601 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     3661 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     3721 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     3781 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     3841 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     3901 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     3961 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     4021 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     4081 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     4141 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataggcta gccgcccgcc
     4201 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tgatatcaga
     4261 tccccgggat gcagaaattg atgatctatt aaacaataaa gatgtccact aaaatggaag
     4321 tttttcctgt catactttgt taagaagggt gagaacagag tacctacatt ttgaatggaa
     4381 ggattggagc tacgggggtg ggggtggggt gggattagat aaatgcctgc tctttactga
     4441 aggctcttta ctattgcttt atgataatgt ttcatagttg gatatcataa tttaaacaag
     4501 caaaaccaaa ttaagggcca gctcattcct cccactcatg atctatagat ctatagatct
     4561 ctcgtgggat cattgttttt ctcttgattc ccactttgtg gttctaagta ctgtggtttc
     4621 caaatgtgtc agtttcatag cctgaagaac gagatcagca gcctctgttc cacatacact
     4681 tcattctcag tattgttttg ccaagttcta attccatcag aagctggtcg agatccggaa
     4741 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgaagaggt
     4801 tcactaggat ccacttttct agctgcccct ttctgtaata gtgaaagttg gtatttaaca
     4861 tttattcatt tttaaaatat ttggaaggtc tgagcttgtg aaaagaaagt ggttggtctg
     4921 aggttggagg aagctgaatg gaatctgacg gttgggagtg gtggaaattg gaaggatacc
     4981 aggaggtatt tgggaaggcc aatggcgtgg ctcctttgag gaaataaaac actaagcatg
     5041 agccggctcc gcctcttctg tctccgcttt catcccaggg cacagagcct tgccttccat
     5101 gctgcccagg gagggcagcc cacggcagcc atgcccctcc ccacctcgct ttcatcatga
     5161 gctcgctccc gagcggccac agcactcatg aatgaagacc ttggggccct tcacagacac
     5221 agatgcagcc agctgtggct ctgaaggccc tggggcccgg gcaccatggt tcacaccttt
     5281 aatcgcagca ctttgggagt ctgggagtta aagaccagcc tcggcaacat agtgagaccc
     5341 cgtctctaca ggaaattaaa tcaggtgtgg tggtgcatgc ctgtagtccc agctacttga
     5401 gggctgaggt gggaggatca cccaagccca agaggtcgag gctgcagtga gctgtgatct
     5461 caccactgca ctccagcctg ggtgacagag caagaccctg tctcaaaaaa aaaaagctgg
     5521 gcgtggtggc tcatgcctgt aatcccagca ctttgggagg ccgaggcggg cggatcacca
     5581 aattagccgg gcatggtggc acatgcctgt aatcccagct actcgggagg ctgaggcagg
     5641 agaattgctt gaacctggga ggcggaggtt gcagtgagct gaaaaaaaaa aaggcagccc
     5701 ccagccgctt gtgttcttga ccagggcccc aggacttggc tcctccagac aagggagttt
     5761 tgtgctgtag atgagggagt tgcccatcgc cgccctagca agtccattcc cacacgacct
     5821 ttccagtggt gatgatgaca gtggcccact cgagcatgca tctagagggc ccaattcgcc
     5881 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     5941 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6001 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6061 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6121 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6181 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6241 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6301 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6361 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6421 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6481 aacgcgaatt ttaacaaaat tcagggcgca agggctgcta aaggaagcgg aacacgtaga
     6541 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga
     6601 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat
     6661 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct
     6721 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct
     6781 gatggcgcag gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     6841 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     6901 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     6961 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     7021 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     7081 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     7141 tgtcatccca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     7201 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     7261 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     7321 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     7381 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     7441 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     7501 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     7561 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     7621 tcttctgaat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7681 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7741 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7801 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7861 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7921 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7981 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8041 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8101 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8161 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8221 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8281 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8341 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8401 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8461 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8521 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8581 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8641 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8701 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8761 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8821 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8881 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8941 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9001 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     9061 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     9121 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     9181 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9241 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9301 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9361 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9421 cgagtcagtg agcgaggaag cggaag
//